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biotin  (Bioss)


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    Bioss biotin
    ( A ) <t>RAGE</t> antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. <t>*RAGEab-biotin:</t> <t>biotin-conjugated</t> RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).
    Biotin, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin/product/Bioss
    Average 90 stars, based on 1 article reviews
    biotin - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "ATP synthase subunit-β down-regulation aggravates diabetic nephropathy"

    Article Title: ATP synthase subunit-β down-regulation aggravates diabetic nephropathy

    Journal: Scientific Reports

    doi: 10.1038/srep14561

    ( A ) RAGE antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. *RAGEab-biotin: biotin-conjugated RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).
    Figure Legend Snippet: ( A ) RAGE antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. *RAGEab-biotin: biotin-conjugated RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).

    Techniques Used: Binding Assay, Labeling, Fluorescence, Enzyme-linked Immunosorbent Assay, Incubation, Transfection, Western Blot, Real-time Polymerase Chain Reaction, Reporter Assay, Luciferase, Activity Assay, Plasmid Preparation



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    ( A ) <t>RAGE</t> antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. <t>*RAGEab-biotin:</t> <t>biotin-conjugated</t> RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).
    Biotin, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin/product/Bioss
    Average 90 stars, based on 1 article reviews
    biotin - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    ( A ) RAGE antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. *RAGEab-biotin: biotin-conjugated RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).

    Journal: Scientific Reports

    Article Title: ATP synthase subunit-β down-regulation aggravates diabetic nephropathy

    doi: 10.1038/srep14561

    Figure Lengend Snippet: ( A ) RAGE antibody binding to RAGE proteins assay in renal proximal tubular cells. The HK-2 cells were treated with the FITC-labeled RAGE antibody (RAGEab-FITC, 10 μg/ml) for 1 hour. The ability of RAGE antibody binding to RAGE proteins was detected by fluorescence ELISA reader. *RAGEab-biotin: biotin-conjugated RAGE antibody. Agarose-streptavidin: streptavidin-conjugated agarose beads. ( B ) HK-2 cells were incubated with AGE-BSA (30 μg/ml) for 24 hours with or without RAGE antibodies (RAGEab, 10 μg/ml) pretreatment for 1 hour. ( C ) HK-2 cells were transiently transfected with ATP5b siRNA (80 nM) for 6 hours and subsequently treated with AGE-BSA (30 μg/ml) for 24 hours. Protein expressions of collagen IV, fibrinogen γ, α-SMA, CTGF, and ATP5b were determined by Western blotting ( B , C ). Quantification of immunoblotting was determined by densitometric analysis. ( D ) The mRNA expressions of CTGF and TGFβ were determined by real-time PCR. HK-2 cells were exposed to AGEs for 24 hours and then subjected to rela-time PCR assay. The mRNA levels were quantified by densitometry and normalized by GAPDH levels. ( E ) The reporter assay for CTGF gene. HK-2 cells were exposed to AGEs for 24 hours with or without ATP5b siRNA transfection. Dual luciferase reporter assay was performed to observe the effect of ATP5b on CTGF transcriptional activity. Data are presented as means ± SEM (n ≥ 5). * P < 0.05, column 4 versus column 3 ( A ); AGE-BSA versus BSA ( B–D ); AGE-BSA/scramble siRNA versus pCTGF vector ( E ). # P < 0.05, column 5 versus column 4 ( A ); AGE-BSA/RAGEab versus AGE-BSA ( B ); AGE-BSA/ATP5b siRNA versus AGE-BSA/scramble siRNA ( C-E ).

    Article Snippet: The supernatant of lysate was incubated with or without biotin-conjugated-RAGE polyclonal antibody (2 μg/ml, #bs-4999R-Biotin, Bioss) in the presence of streptavidin-conjugated agarose beads (#S1638, Sigma-Aldrich) at 4 °C overnight.

    Techniques: Binding Assay, Labeling, Fluorescence, Enzyme-linked Immunosorbent Assay, Incubation, Transfection, Western Blot, Real-time Polymerase Chain Reaction, Reporter Assay, Luciferase, Activity Assay, Plasmid Preparation